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Windows 98SE / ME / 2000 / XP (Pro, Home, and Media Center) / Vista operating system
32 MB of free drive space
CD-ROM or DVD-ROM
32 MB of memory
For Win98SE, USB 2.0 support is required for operation (regardless of USB or serial support being purchased). USB 2.0 is built-into Windows 2000 and later.
If it will run XP, you're good. It will run on the older OS's but some of them have driver problems with USB ports. XP has much better native USB support so I always recommend at least that OS.