Yes. And run quite well, too.

The problem is that B-100 biodiesel clouds around 30 degrees, so if you live in a colder part of the country you'll need to use an anti-gel additive or mix it with maybe 50% regular pump diesel.

I ran a B-50 mix last week down to 5 degreeðM
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”´SB riment and my truck never ran smoother and quieter. But because of cost I generally run B-20 year-round.